| 1. | The transient expression of mgfp4 in rice calli
4在水稻愈伤组织中的瞬时表达 |
| 2. | Transient expression of fibrillin promoter in protoplasts of arabidopsis thaliana and brassica napus
操纵子在甘蓝型油菜和拟南芥中的瞬时表达 |
| 3. | Acetosyringone ( as ) can increase the gus gene transient expression when it was added in the suspending medium
在菌液中添加as可以提高gus基因的瞬时表达。 |
| 4. | The results were that transient expression had not obtained after transformating dry seeds implanted
结果表明：直接注入干种子后进行转化，未得到瞬间表达的结果。 |
| 5. | But if 2 - day - seedling was implanted , and then dipped into dna solution , the transformation effect was increased evidently . 10 % transient expression was obtainted
而注入玻璃化保护后的2天龄幼苗，再进行转化，转化效果明显提高，得到了大约10 %的瞬间表达。 |
| 6. | The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7
瞬时转染cos刁细胞后，通过荧光显微镜观察、间接免疫荧光检测、免疫组化检测证实了scfv融合蛋白的表达。 |
| 7. | The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences
以lacz为报告基因的瞬间表达实验结果表明，长度分别为306bp和302bp的crtw和crtz5 '上游侧翼序列具有很强的启动转录活性，提示两段序列包含了启动子的结构。 |
| 8. | In transgenic tobacco plants , the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1 . 2 kb pdf1 . 2 promoter fused to a gus reporter gene was induced by meja
( 1 )从拟南芥基因组中扩增出长度约为1200bp的pdf1 . 2启动子，与gus构建的融合基因在烟草中的表达受meja诱导。 |
| 9. | Sonication - assisted agrobacterium - mediated transformation ( saat ) can increase the efficiency of transformation . 2s sonication can enhance the gus gene transient expression and induction of resistant buds without leaves incision
头孢毒素( cef )对不定芽的诱导有一定的抑制作用，哌拉西林钠( pip )对不定芽的诱导抑制作用稍弱，以pip400mg / l作为抑菌生长浓度。 |
| 10. | ( 3 ) on the basis of the deletion analysis , three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element . transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation . loss - of - function experiment , using transient expression analysis of gus reporter genes , confirmed that gcc box act as an essential element to respond ja signaling in pdf1 . 2 promoter
( 3 )在缺失突变的基础上，通过对gccbox及其相邻的上下游六个碱基进行取代突变，将突变启动子与gus构建融合基因，在烟草中受heja诱导的瞬时表达结果表明， h1和m3的突变对该启动子应答ja信号的影响很小，而m2 ( gccbox的突变)则几乎使该启动子应答ja信号的功能完全丧失，所以gccbox是该启动子中应答ja信号的必需元件。 |
